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Amino Acid Sequences of Three Higher Plant EPSP Synthases
Question What are the amino acid sequences of three (Arabidopsis, tomato and tobacco) higher plant EPSP synthases? Use the NCBI site (https://www.ncbi.nlm.nih.gov/) and the ‘Protein’ search for these. Type in the enzyme name and the species. Quote the accession numbers when you find the ... Read More
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Molecular Masses of Proteins and Transit Peptide Sequences
Question What are the molecular masses of these proteins? Can you detect any transit peptide sequences for these proteins? Summary This question belongs to biology and discusses about the molecular masses of proteins and transit peptide sequences for these proteins. Total word count: 163 ... Read More
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Amino Acid Sequence of EPSP Synthase from Agrobacterium Strain CP4
Question What is the amino acid sequence of EPSP synthase from Agrobacterium strain CP4 (accession number Q9R4E4)? Copy the FASTA sequence in to your Word document. What is its molecular mass? Use NCBI for this. Can you detect a transit peptide sequence for this protein? Summary This question bel ... Read More
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Amino Acid Sequences of the Higher Plant and Bacterial EPSP Synthases
Question Compare the amino acid sequences of the higher plant and bacterial EPSP synthases. To do this, you need to convert the amino acid sequences into a ‘Fasta’ format. Go to NCBI, choose the FASTA format from ‘display’. Then paste in each sequence, including the part sta ... Read More
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Amino acid Sequences must Account for Resistance to Glyphosate
Question What are the major differences? Are the sequences a similar length? If not why are they different? Differences in the amino acid sequences must account for resistance to glyphosate. You will find several differences, but we know that the key changes are around amino acid 100. Look at this ... Read More
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Genetic Manipulations used to Engineer glyphosate Resistance into Crops
Question What other genetic manipulations are used to engineer glyphosate resistance into crops? (See the abstract of Klee et al (1987) Mol Gen Genet 210: 437-442). Summary This question belongs to biology and discusses about genetic manipulations that are used to engineer glyphosate resistance i ... Read More
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Routine Use of a Genetic Marker that is not Currently used in CODIS
Question Advocate for routine use of a genetic marker that is not currently used in CODIS. The marker can be any genetic element The marker can be used to aid in any area of forensic biology/DNA analysis such as identification of a body fluid, provide information about the persons appeara ... Read More
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Relation between Branches as given in the Cladogram Diagram
Question Based on your cladogram, is A2 more closely related to A6 or A7 (or is it equally related to both)? Is A2 more closely related to A3 or A1 (or is it equally related to both). Explain your answers. Summary This question belongs to biology and discusses about relation between branches as ... Read More
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Monophyletic Relation between Branches as given in the Cladogram Diagram
Question Is a clade that included only A1, A2 and A4 be monophyletic? Why / why not? Summary This question belongs to biology and discusses about monophyletic relation between branches as given in the cladogram diagram. Total word count: 21 ... Read More
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Synapomorphy for the Smallest Monophyletic Clade
Question Define a synapomorphy for the smallest monophyletic clade that includes A3 and A2. Summary This question belongs to biology and discusses about synapomorphy for the smallest monophyletic clade as given in the cladogram diagram. Total word count: 17 ... Read More
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Spiny---bodied two—Arms Clade
Question (Above image) Centuries ago, scientists would group together A4, A5 and A6 as “spiny---bodied two—Arms”. What is the term for the type of clade represented by “Spiny----bodied two----Arms”? Summary This question belongs to biology and discusses about spiny ... Read More
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Character Evolved First: Spines on the Arms or Two Ovaries
Question Which character evolved first: spines on the arms or two ovaries? Summary This question belongs to biology and discusses about character evolved first as given in the cladogram diagram. Total word count: 14 ... Read More
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